Protein ion-exchange chromatography on a biomacromolecule-immobilized monolithic cryogel

Immobilized Metal Ion Chromatography

1974 First use of immobilized chelators to isolate metalloproteins 1975 First description of general technique (IMAC) using IDA 1983 Introduction of high performance on silica based media 1986 Use of Fe3# chelates to purify phosphoproteins 1987 Introduction of NTA 1988 Introduction of genetically engineered His tags 1992 Introduction of TREN 1998 Introduction of TACN Freeze NH (1995) Lectin afR...

Monitoring Protein PEGylation with Ion Exchange Chromatography

Ion Exchange Chromatography

The separation and purification of various elements by ion exchange chromatography takes advantage of the variation of the electrostatic bond energies of ions in solution. Thus, the two main controlling factors in ion exchange chromatography are the ionic charge (z) and the ionic radius (r). These two are combined in the ionic potential – z/r – that can be used to compare relative electrostatic...

Displacement Chromatography on Synthetic Ion - exchange Resins

In Part 1 of this series of papers (Partridge & Westall, 1949) it was shown that, whilst the baseexchange resin Zeo Karb 215 may be used with success for the separation of the neutral and acidic amino-acids, certain difficulties arise when the separation of basic substances is attempted. These difficulties were traced to the phenolic hydroxyl groups of the resin. Resins of the sulphonated pheno...

Ion Exchange Chromatography - An Overview

Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that t...